LOCUS       40924_315       13067 bp DNA     circular SYN 17-DEC-2018
DEFINITION  BAC cloning vector attB-P[acman]-ApR-F-2-5-attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Venken KJ, He Y, Hoskins RA, Bellen HJ.
  TITLE     P[acman]: a BAC transgenic platform for targeted insertion of large 
            DNA fragments in D. melanogaster
  JOURNAL   Science 314 (5806), 1747-1751 (2006)
  PUBMED    17138868
REFERENCE   2  (bases 1 to 13067)
  AUTHORS   Venken KJT., He Y, Hoskins RA, Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2006) Molecular and Human Genetics, Baylor College
            of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 13067)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13067)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2006"; volume: "314"; issue: "5806"; pages: "1747-1751"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-NOV-2006) Molecular and Human Genetics, Baylor College of 
            Medicine, One Baylor Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(244..717)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(793..1761)
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1764..2936)
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    complement(3262..3512)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3518..4270)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(4361..4580)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4659..5372
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6236..6338
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        6339..6410
                     /label=AmpR promoter
     CDS             6411..7268
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     protein_bind    7676..7745
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(7960..8192)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     8199..8215
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8225..8243
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8287..8361
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(8380..8398)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8434..12449
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
     CDS             join(8918..8989,9391..9664,9739..10393,10455..10770,
                     10974..11105,11176..11790)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    complement(12450..13035)
                     /label=P element 5' end
ORIGIN
        1 tcgacagcga cacacttgca tcggatgcag cccggttaac gtgccggcac ggcctgggta
       61 accaggtatt ttgtccacat aaccgtgcgc aaaatgttgt ggataagcag gacacagcag
      121 caatccacag caggcataca accgcacacc gaggttactc cgttctacag gttacgacga
      181 catgtcaata cttgcccttg acaggcattg atggaatcgt agtctcacgc tgatagtctg
      241 atcgacaata caagtgggac cgtggtccca gaccgataat cagaccgaca acacgagtgg
      301 gatcgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
      361 aataatcaga ccgacgatac gagtgggacc gtggttccag actaataatc agaccgacga
      421 tacgagtggg accgtggtcc cagactaata atcagaccga cgatacgagt gggaccatgg
      481 tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccagt ctgattatca
      541 gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg
      601 ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagtc
      661 tgattatcag accgacgata caagtggaac agtgggccca gagagaatat tcaggccagt
      721 tatgctttct ggcctgtaac aaaggacatt aagtaaagac agataaacgt agactaaaac
      781 gtggtcgcat cagggtgctg gcttttcaag ttccttaaga atggcctcaa ttttctctat
      841 acactcagtt ggaacacgag acctgtccag gttaagcacc attttatcgc ccttatacaa
      901 tactgtcgct ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg atgcagatga
      961 cgttttaagc acagaagtta aaagagtgat aacttcttca gcttcaaata tcaccccagc
     1021 ttttttctgc tcatgaaggt tagatgcctg ctgcttaagt aattcctctt tatctgtaaa
     1081 ggctttttga agtgcatcac ctgaccgggc agatagttca ccggggtgag aaaaaagagc
     1141 aacaactgat ttaggcaatt tggcggtgtt gatacagcgg gtaataatct tacgtgaaat
     1201 attttccgca tcagccagcg cagaaatatt tccagcaaat tcattctgca atcggcttgc
     1261 ataacgctga ccacgttcat aagcacttgt tgggcgataa tcgttaccca atctggataa
     1321 tgcagccatc tgctcatcat ccagctcgcc aaccagaaca cgataatcac tttcggtaag
     1381 tgcagcagct ttacgacggc gactcccatc ggcaatttct atgacaccag atactcttcg
     1441 accgaacgcc ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat catccagtgc
     1501 gtcctcagta agcagctcct ggtcacgttc attacctgac catacccgag aggtcttctc
     1561 aacactatca ccccggagca cttcaagagt aaacttcaca tcccgaccac atacaggcaa
     1621 agtaatggca ttaccgcgag ccattactcc tacgcgcgca attaacgaat ccaccatcgg
     1681 ggcagctggt gtcgataacg aagtatcttc aaccggttga gtattgagcg tatgttttgg
     1741 aataacaggc gcacgcttca ttatctaatc tcccagcgtg gtttaatcag acgatcgaaa
     1801 atttcattgc agacaggttc ccaaatagaa agagcatttc tccaggcacc agttgaagag
     1861 cgttgatcaa tggcctgttc aaaaacagtt ctcatccgga tctgaccttt accaacttca
     1921 tccgtttcac gtacaacatt ttttagaacc atgcttcccc aggcatcccg aatttgctcc
     1981 tccatccacg gggactgaga gccattacta ttgctgtatt tggtaagcaa aatacgtaca
     2041 tcaggctcga accctttaag atcaacgttc ttgagcagat cacgaagcat atcgaaaaac
     2101 tgcagtgcgg aggtgtagtc aaacaactca gcaggcgtgg gaacaatcag cacatcagca
     2161 gcacatacga cattaatcgt gccgataccc aggttaggcg cgctgtcaat aactatgaca
     2221 tcatagtcat gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg atcggtgggc
     2281 agtttacctt catcaaattt gcccattaac tcagtttcaa tacggtgcag agccagacag
     2341 gaaggaataa tgtcaagccc cggccagcaa gtgggcttta ttgcataagt gacatcgtcc
     2401 ttttccccaa gatagaaagg caggagagtg tcttctgcat gaatatgaag atctggtacc
     2461 catccgtgat acattgaggc tgttccctgg gggtcgttac cttccacgag caaaacacgt
     2521 agccccttca gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt gtaaacgcca
     2581 cctttatggg cagcaacccc gatcaccggt ggaaatacgt cttcagcacg tcgcaatcgc
     2641 gtaccaaaca catcacgcat atgattaatt tgttcaattg tataaccaac acgttgctca
     2701 acccgtcctc gaatttccat atccgggtgc ggtagtcgcc ctgctttctc ggcatctctg
     2761 atagcctgag aagaaacccc aactaaatcc gctgcttcac ctattctcca gcgccgggtt
     2821 attttcctcg cttccgggct gtcatcatta aactgtgcaa tggcgatagc cttcgtcatt
     2881 tcatgaccag cgtttatgca ctggttaagt gtttccatga gtttcattct gaacatcctt
     2941 taatcattgc tttgcgtttt tttattaaat cttgcaattt actgcaaagc aacaacaaaa
     3001 tcgcaaagtc atcaaaaaac cgcaaagttg tttaaaataa gagcaacact acaaaaggag
     3061 ataagaagag cacatacctc agtcacttat tatcactagc gctcgccgca gccgtgtaac
     3121 cgagcatagc gagcgaactg gcgaggaagc aaagaagaac tgttctgtca gatagctctt
     3181 acgctcagcg caagaagaaa tatccaccgt gggaaaaact ccaggtagag gtacacacgc
     3241 ggatagccaa ttcagagtaa taaactgtga taatcaaccc tcatcaatga tgacgaacta
     3301 acccccgata tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg tgacaaactg
     3361 ccctcaaatt tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa tccatgcagg
     3421 ctgaaggaaa cagcaaaact gtgacaaatt accctcagta ggtcagaaca aatgtgacga
     3481 accaccctca aatctgtgac agataaccct cagactatcc tgtcgtcatg gaagtgatat
     3541 cgcggaagga aaatacgata tgagtcgtct ggcggccttt ctttttctca atgtatgaga
     3601 ggcgcattgg agttctgctg ttgatctcat taacacagac ctgcaggaag cggcggcgga
     3661 agtcaggcat acgctggtaa ctttgaggca gctggtaacg ctctatgatc cagtcgattt
     3721 tcagagagac gatgcctgag ccatccggct tacgatactg acacagggat tcgtataaac
     3781 gcatggcata cggattggtg atttcttttg tttcactaag ccgaaactgc gtaaaccggt
     3841 tctgtaaccc gataaagaag ggaatgagat atgggttgat atgtacactg taaagccctc
     3901 tggatggact gtgcgcacgt ttgataaacc aaggaaaaga ttcatagcct ttttcatcgc
     3961 cggcatcctc ttcagggcga taaaaaacca cttccttccc cgcgaaactc ttcaatgcct
     4021 gccgtatatc cttactggct tccgcagagg tcaatccgaa tatttcagca tatttagcaa
     4081 catggatctc gcagataccg tcatgttcct gtagggtgcc atcagatttt ctgatctggt
     4141 caacgaacag atacagcata cgtttttgat cccgggagag actatatgcc gcctcagtga
     4201 ggtcgtttga ctggacgatt cgcgggctat ttttacgttt cttgtgattg ataaccgctg
     4261 tttccgccat gacagatcca tgtgaagtgt gacaagtttt tagattgtca cactaaataa
     4321 aaaagagtca ataagcaggg ataactttgt gaaaaaacag cttcttctga gggcaatttg
     4381 tcacagggtt aagggcaatt tgtcacagac aggactgtca tttgagggtg atttgtcaca
     4441 ctgaaagggc aatttgtcac aacaccttct ctagaaccag catggataaa ggcctacaag
     4501 gcgctctaaa aaagaagatc taaaaactat aaaaaaaata attataaaaa tatccccgtg
     4561 gataagtgga taaccccaag ggaagttttt tcaggcatcg tgtgtaagca gaatatataa
     4621 gtgctgttcc ctggtgcttc ctcgctcact cgaaattccc ctttccgacg ctcaccgggc
     4681 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc
     4741 cgtcgaagcc gtgtgcgaga caccgcggcc ggccgccggc gttgtggata cctcgcggaa
     4801 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     4861 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     4921 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     4981 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     5041 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     5101 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     5161 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     5221 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     5281 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     5341 ggccgcgaac ggcctcaccc caaaaatggc aggggatctc gagggcttcg ccctgtcgct
     5401 cgactgcggc gagcactact ggctgtaaaa ggacagacca catcatggtt ctgtgttcat
     5461 taggttgttc tgtccattgc tgacataatc cgctccactt caacgtaaca ccgcacgaag
     5521 atttctattg ttcctgaagg catattcaaa tcgttttcgt taccgcttgc aggcatcatg
     5581 acagaacact acttcctata aacgctacac aggctcctga gattaataat gcggatctct
     5641 acgataatgg gagattttcc cgactgtttc gttcgcttct cagtggataa cagccagctt
     5701 ctctgtttaa cagacaaaaa cagcatatcc actcagttcc acatttccat ataaaggcca
     5761 aggcatttat tctcaggata attgtttcag catcgcaacc gcatcagact ccggcatcgc
     5821 aaactgcacc cggtgccggg cagccacatc cagcgcaaaa accttcgtgt agacttccgt
     5881 tgaactgatg gacttatgtc ccatcaggct ttgcagaact ttcagcggta taccggcata
     5941 cagcatgtgc atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa cagagaacgt
     6001 cacaccgtca gcagcagcgg cggcaaccgc ctccccaatc caggtcctga ccgttctgtc
     6061 cgtcacttcc cagatccgcg ctttctctgt ccttcctgtg cgacggttac gccgctccat
     6121 gagcttatcg cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg
     6181 gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc
     6241 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     6301 ttgagttatc gagattttca ggagctaagg aagctaaaca ttcaaatatg tatccgctca
     6361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6541 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6721 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6781 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7021 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7141 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7261 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7501 cagcggtggt ttgtttgccg gatcaagagc taccaactct tttttttaag gcagttattg
     7561 gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag
     7621 aaattcgatg ataagctgtc aaacatgaga attggtcgac gatgtaggtc acggtctcga
     7681 agccgcggtg cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccacctcac
     7741 ccatctggtc catcatgatg aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc
     7801 ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg
     7861 gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg
     7921 tcacggcggg catgtcgatc acgatgagaa tggccagacc atgatgaaat aacataaggt
     7981 ggtcccgtcg gcaagagaca tccacttaac gtatgcttgc aataagtgcg agtgaaagga
     8041 atagtattct gagtgtcgta ttgagtctga gtgagacagc gatatgattg ttgattaacc
     8101 cttagcatgt ccgtggggtt tgaattaact cataatatta attagacgaa attattttta
     8161 aagttttatt tttaataatt tgcgagtacg caaccggtgt aaaacgacgg ccagtgagcg
     8221 cgcgtaatac gactcactat agggcgaatt ggagctcgtt taaacctcga gcggtccgtt
     8281 atcgatggcg cgccggcctt aatggccgcg gccgcattta aatgtcgact taattaaggc
     8341 cggccgcgat cgcgcccggg cggtacccag cttttgttcc ctttagtgag ggttaattgc
     8401 gcgcttggcg taatcatggt catagctgtt tccgaattct agtatgtatg taagttaata
     8461 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
     8521 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
     8581 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     8641 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     8701 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg
     8761 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
     8821 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
     8881 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     8941 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
     9001 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
     9061 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt
     9121 gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat
     9181 atcatgatca agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata
     9241 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
     9301 tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta
     9361 gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc
     9421 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg
     9481 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
     9541 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
     9601 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
     9661 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
     9721 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
     9781 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
     9841 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
     9901 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
     9961 ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc
    10021 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
    10081 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
    10141 cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg
    10201 gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
    10261 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
    10321 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
    10381 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct
    10441 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
    10501 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
    10561 atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc
    10621 aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
    10681 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
    10741 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
    10801 aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac
    10861 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa
    10921 tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
    10981 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata
    11041 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
    11101 taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac tttctaatga
    11161 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
    11221 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt
    11281 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
    11341 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
    11401 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
    11461 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
    11521 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
    11581 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
    11641 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
    11701 ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
    11761 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
    11821 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
    11881 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
    11941 cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc
    12001 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
    12061 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
    12121 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
    12181 cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt
    12241 catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat
    12301 atatttgtat atatttccct cacagctata tttattctaa tttaatatta tgacttttta
    12361 aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca
    12421 tccagtgttt gttccttgtg tagatgcatg ctgctgctct aaacgacgca tttcgtactc
    12481 caaagtacga attttttccc tcaagctctt attttcatta aacaatgaac aggacctaac
    12541 gcacagtcac gttattgttt acataaatga ttttttttac tattcaaact tactctgttt
    12601 gtgtactccc actggtatag ccttctttta tcttttctgg ttcaggctct atcactttac
    12661 taggtacggc atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag
    12721 ccttccactg cgaatcatta aagtgggtat cacaaatttg ggagttttca ccaaggctgc
    12781 acccaaggct ctgctcccac aattttctct taatagcaca cttcggcacg tgaattaatt
    12841 ttactccagt cacagctttg cagcaaaatt tgcaatattt catttttttt tattccacgt
    12901 aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct caacaagcaa
    12961 acgtgcactg aatttaagtg tatacttcgg taagcttcgg ctatcgacgg gaccacctta
    13021 tgttatttca tcatgggcca gacccacgta gtccagacta gtggatc
//
